we’ll see if it can hold it all day.
So we start the day punching up. The shares will show us the way.
we’ll see if it can hold it all day.
So we start the day punching up. The shares will show us the way.
Here is the actual report and goes on past what I copied just in case it disappeared. It has a cookies in front of it you can scroll around it.
bitor of SARS coronavirus infection and spread
* Martin J Vincent, Eric Bergeron, Suzanne Benjannet, Bobbie R Erickson, Pierre E Rollin, Thomas G Ksiazek, Nabil G Seidah & Stuart T Nichol -Show fewer authors
Virology Journal
volume
2, Article number: 69 (2005) Cite this article
Abstract
Background
Severe acute respiratory syndrome (SARS) is caused by a newly discovered coronavirus (SARS-CoV). No effective prophylactic or post-exposure therapy is currently available.
Results
We report, however, that chloroquine has strong antiviral effects on SARS-CoV infection of primate cells. These inhibitory effects are observed when the cells are treated with the drug either before or after exposure to the virus, suggesting both prophylactic and therapeutic advantage. In addition to the well-known functions of chloroquine such as elevations of endosomal pH, the drug appears to interfere with terminal glycosylation of the cellular receptor, angiotensin-converting enzyme 2. This may negatively influence the virus-receptor binding and abrogate the infection, with further ramifications by the elevation of vesicular pH, resulting in the inhibition of infection and spread of SARS CoV at clinically admissible concentrations.
Conclusion
Chloroquine is effective in preventing the spread of SARS CoV in cell culture. Favorable inhibition of virus spread was observed when the cells were either treated with chloroquine prior to or after SARS CoV infection. In addition, the indirect immunofluorescence assay described herein represents a simple and rapid method for screening SARS-CoV antiviral compounds.
Background
Severe acute respiratory syndrome (SARS) is an emerging disease that was first reported in Guangdong Province, China, in late 2002. The disease rapidly spread to at least 30 countries within months of its first appearance, and concerted worldwide efforts led to the identification of the etiological agent as SARS coronavirus (SARS-CoV), a novel member of the family Coronaviridae [1]. Complete genome sequencing of SARS-CoV [2, 3] confirmed that this pathogen is not closely related to any of the previously established coronavirus groups. Budding of the SARS-CoV occurs in the Golgi apparatus [4] and results in the incorporation of the envelope spike glycoprotein into the virion. The spike glycoprotein is a type I membrane protein that facilitates viral attachment to the cellular receptor and initiation of infection, and angiotensin-converting enzyme-2 (ACE2) has been identified as a functional cellular receptor of SARS-CoV [5]. We have recently shown that the processing of the spike protein was effected by furin-like convertases and that inhibition of this cleavage by a specific inhibitor abrogated cytopathicity and significantly reduced the virus titer of SARS-CoV [6].
Due to the severity of SARS-CoV infection, the potential for rapid spread of the disease, and the absence of proven effective and safe in vivo inhibitors of the virus, it is important to identify drugs that can effectively be used to treat or prevent potential SARS-CoV infections. Many novel therapeutic approaches have been evaluated in laboratory studies of SARS-CoV: notable among these approaches are those using siRNA [7], passive antibody transfer [8], DNA vaccination [9], vaccinia or parainfluenza virus expressing the spike protein [10, 11], interferons [12, 13], and monoclonal antibody to the S1-subunit of the spike glycoprotein that blocks receptor binding [14]. In this report, we describe the identification of chloroquine as an effective pre- and post-infection antiviral agent for SARS-CoV. Chloroquine, a 9-aminoquinoline that was identified in 1934, is a weak base that increases the pH of acidic vesicles. When added extracellularly, the non-protonated portion of chloroquine enters the cell, where it becomes protonated and concentrated in acidic, low-pH organelles, such as endosomes, Golgi vesicles, and lysosomes. Chloroquine can affect virus infection in many ways, and the antiviral effect depends in part on the extent to which the virus utilizes endosomes for entry. Chloroquine has been widely used to treat human diseases, such as malaria, amoebiosis, HIV, and autoimmune diseases, without significant detrimental side effects [15]. Together with data presented here, showing virus inhibition in cell culture by chloroquine doses compatible with patient treatment, these features suggest that further evaluation of chloroquine in animal models of SARS-CoV infection would be warranted as we progress toward finding effective antivirals for prevention or treatment of the disease.
Results
Preinfection chloroquine treatment renders Vero E6 cells refractory to SARS-CoV infection
In order to investigate if chloroquine might prevent SARS-CoV infection, permissive Vero E6 cells [1] were pretreated with various concentrations of chloroquine (0.1–10 μM) for 20–24 h prior to virus infection. Cells were then infected with SARS-CoV, and virus antigens were visualized by indirect immunofluorescence as described in Materials and Methods. Microscopic examination (Fig. 1A) of the control cells (untreated, infected) revealed extensive SARS-CoV-specific immunostaining of the monolayer. A dose-dependant decrease in virus antigen-positive cells was observed starting at 0.1 μM chloroquine, and concentrations of 10 μM completely abolished SARS-CoV infection. For quantitative purposes, we counted the number of cells stained positive from three random locations on a slide. The average number of positively stained control cells was scored as 100% and was compared with the number of positive cells observed under various chloroquine concentrations (Fig. 1B). Pretreatment with 0.1, 1, and 10 μM chloroquine reduced infectivity by 28%, 53%, and 100%, respectively. Reproducible results were obtained from three independent experiments. These data demonstrated that pretreatment of Vero E6 cells with chloroquine rendered these cells refractory to SARS-CoV infection.
https://virologyj.biomedcentral.com/articles/10.1186/1743-422X-2-69
Coronavirus vaccine
The NIH partnered with Moderna in 2020 during the COVID-19 pandemic to develop a vaccine. The final phase of testing began on 27 July with 30,000 volunteers assigned to two groups — one receiving the mRNA-1273 vaccine and the other receiving salt water injections with 150 cases of COVID-19 administered amongst the participants.[35]
https://en.m.wikipedia.org/wiki/National_Institutes_of_Health
Yes I read that and also from a site on Fauci and NIH they partnered with a company making a vaccine. Gates has money in a lot of them.
By Armstrong
There are NO COINCIDENCES in life!George Soros, remember that name? If you vote Biden you never will!WELL, WELL!! Isn’t it just an interesting coincidence that Remdesivir is made by Gilead and is the “CURE” for COVID-19….and here’s where it gets interesting: China holds the patent on the drug through an agreement with Gilead’s drug patent sharing subsidiary branch called UNITAID, who has an office near Wuhan, and you’ll never guess who are the main financial investors in UNITAID….none other than George Soros, Bill & Melinda Gates, and WHO. I know what you’re thinking! It’s just all a coincidence isn’t it! Oh, don’t let me forget the other coincidence, that Gilead and UNITAID were financial supporters of Hillary Clinton. Oh, silly me, I forgot one more coincidence, Fauci was the one authorizing millions to be sent to The Wuhan Institute of Virology specifically for the “study” of Coronaviruses……I sure am thankful it’s all just coincidences! Nothing to see here, just keep moving along….WOW! No wonder Fauci slapped down hydroxychloroquine which has a 92% success rate…he was told to!! Fauci’s wife also works for Gilead.The Best Coincidences are always found when you follow the money..! ! !I don’t know… does it make you ask questions? Do your own research… could all just be coincidence?!?!Here is a link to substantiate this….
Something strange going on with your computer. I feel for you because I’m about as knowledgeable about computers as a certain group of politicians is on the economy. It would be very frustrating to say the least. Just lucky I’m not neurotic or would of had a bunch of broken ones by now.
I got a nice little iPad from metro you only pay a extra 15 a month with unlimited data you don’t need WiFi for giving me no problems but still need to work on navigation. I’m watching It’s a Mad Mad Mad World on it for background. Old comedy with a bunch of comedians in it with name kinda fitting right now. I read comedians all over wanting to be in on it to get a part even Jerry Lewis so he got a small part including others. Young these days probably wouldn’t recognized them even he three stooges as firemen.
Thanks Ororeef, it appears it’s just my own glitch. I’m trying the Brave browser. They say they have no ads. If that’s true it’ll be a blessing.
Thanks for your input.
NO problems with FIREFOX ..been using it for years ..win 10
also have two laptops with UBUNTU LINUX, FIREFOX no problems
Newtogold, it only happens on Firefox and I agree. Time to trash Firefox. I was only trying to see if it was strictly my problem, or if it was happening to other tenters too.
Goldie, thanks for the summary, but I was able to watch your video using Microsoft Edge. Funny thing though, when I went back to Firefox browser and refreshed it for new messages, Firefox browser had changed your correct video to the same as my wrong video, instead of correcting my video to the one you posted. Anyway too hard to explain clearly.
I am NOT recommending any browsers and I’ve been reluctant to use Microsoft Edge due to its tie to Microsoft, and same reason for Google Chrome due to its tie to Google….. and both those browsers are the ones that try to get you to install them when you install new programs or update old programs if you don’t uncheck the install box. If I can figure how to use it I’ll try a browser called Brave which is supposed to be independent like I thought Firefox was.
Anybody have any other browser suggestions???? Thanks if you do from Silverngold. :o)
In case you can’t see it here’s a few things she pointed out I write down for you then added some at the bottom. She said A Lot more than what I wrote so worth a listen to pass on to the young being brainwashed into their own extinction.
Do you think there is no racism or evil beyond our border progressives want to open allowing in all their ancestors enemies that wanted to destroy or conquer them for their children’s sake? Do you think when blacks get together they worry about the white mans fate or dwindling numbers when they are already a minority in the world? Do they know what they’re voting for when the vote for bigger government and upsurping the constitution that affects all free lives?
WhatI added:
Do these white progressives know what their voting for when they vote for matters of inclusion when it excludes them?
Do white progressives stand up for censorship when it’s a one way censorship excluding them?
Do progressives attack people when their freedom of religion is attacked when they refuse to promote gay life in a birthday cake when it goes against their religion then say it’s not about anti religion?
Do they believe certain progressive women’s groups it’s not anti men when they will uses false accusations of sexual assaults without proof to destroy them for personal or political gain?
Try a different browser and see if you get the same results. I have Firefox and it opens no problem. Maybe it’s your provider like Comcast for example, doing something if it happens on another browser. Keep spreading the word SNG. Good luck with this mysterious event.
She ripped them good.
https://youtu.be/7drzLBsrE9c
IN RESPONSE TO ALL THE RECENT E-MAILS ABOUT MY DOG: I AM SICK AND TIRED
OF ANSWERING QUESTIONS ABOUT HIM.
YES, HE BIT 6 PEOPLE WEARING TRUDEAU T-SHIRTS…
4 PEOPLE WEARING MORNEAU T-SHIRTS…
2 CAR DRIVERS WITH “SUPPORT THE LIBERAL PARTY” BUMPER STICKERS…
9 TEENAGERS WITH PANTS HANGING PAST THE CRACKS OF THEIR BUMS…
SO FOR THE LAST TIME…
THE DOG IS NOT FOR SALE!
AND NO, I DO NOT APPROVE OF HIS SMOKING POT, BUT HE SAYS IT HELPS
TO GET THE BAD TASTE OUT OF HIS MOUTH.
“Arbitragers can buy long August and sell short December to collect $21 dollars per ounce.
One reason I can think of why the spreads persist, is because bullion banks are currently less active on the COMEX. Previously, bullion banks—having access to cheap funding—often performed the arbitrage trades.”
A Record Amount Of Physical Gold Was Just Delivered On COMEX, Here’s Why
A very well orchestrated plan, or a unimaginable set of events that just fell into place … with the United States front and center. You tell me!!
Scare people with a virus, force them to wear masks and place them in quarantine.
Count the number of dead every second of every day, in every News Headline. By the way, ninety-nine and eight-tenths of the people who get the virus, recover. About one to two tenths of one percent who get the virus, die. Most all of them have other medical problems. Did you catch that ? Less than 1/2 of a percent die.
Close businesses = 35,000,000+ instantly unemployed.
Remove entertainment and prohibit Recreation; Closing parks, gyms, bars, restaurants, sports.
No dating. No touching. Isolate people. Dehumanize them.
Close Temples and Churches, prohibit worship. Create a vacuum and let depression, anxiety, hopelessness and desperation set in.
Then… ignite hatred and civil unrest, creating Civil War.
Empty the prisons because of the virus and fill the streets with criminals.
Send in Antifa to vandalize property, as if they are freedom fighters. Undermine the law, Riot, Loot and Attack all Law Enforcement, but tell government to order a stand-down.
Then… Defund Law Enforcement and abolish Police.
yes, that is a good one. Trump probably didn’t get a flu shot, that’s why he could wear that mask!
BTW – that picture reminds me – ANYONE SEEN COMMISH LATELY??
Haven’t seen him since the site went down.
Heading to Florida? Hope all Floridians are safe. Talk about surges hitting the coast.
Hope Florida is secure and being watched over.
What an amazing man you have for President, and he certainly does not deserve the way he has been treated during his presidency. I have only watched the first episode which covers his “early” years, but I’m blown away by his self confidence and accomplishments during his early years. IMO he puts all these career politicians to shame. While most of them have sponged off of tax payers all or most of their lives, Trump actually went out into the business world and accomplished amazing things. I encourage everyone to watch it if you can. You’ll come away with a lot more admiration and respect for your POTUS.
i just got back from grocery shopping, and i saw my first biden for president yard sign. there were a couple of dozen trump – pence signs up (including mine at the end of my driveway) as there have been for several weeks. this is a traditionally democratic county – at least on the local level. either sleepy joe is a little retarded in getting his ground game started, or there’s minimal enthusiasm among his base. it’s early yet, but it looks like trump will have no trouble in this part of florida – possibly excepting leon county (college town and lots of entrenched state employees).